Application
Western blot
Sample
Human Cell lysate - whole cell (Burkitt's lymphoma line)
Loading amount
50000 cells
Specification
Burkitt's lymphoma line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% milk/PBS
Secondary antibody
Name
Non-Abcam antibody was used: Dako P0163 rabbit anti-sheep HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 160 kDa Non-specific: 98, 100, 130 kDa
Positive control
BL31 WT - Burkitt's lymphoma cell line latently infected with EBV
Negative control
BL31 - uninfected Burkitt's lymphoma cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 14 2010