保存方法Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
バッファーPreservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab53360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/1000.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
機能May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
組織特異性Predominantly expressed in brain and muscle.
配列類似性Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.
ドメインComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
細胞内局在Cytoplasm > cytoskeleton. Associated with the microtubule network.
- Information by UniProt
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 8 minutes
Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Pavez M et al. STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones. J Neurosci N/A:N/A (2019). Read more (PubMed: 31023836) »
- Fassier C et al. Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics. J Cell Biol 217:1719-1738 (2018). Read more (PubMed: 29535193) »