Anti-EAAT3 抗体 [EPR6774(B)] (ab124802)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6774(B)] to EAAT3
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-EAAT3 antibody [EPR6774(B)]
EAAT3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6774(B)] to EAAT3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.
-
ポジティブ・コントロール
- WB: Human fetal brain, human brain and human testis lysates . IHC-P: Human kidney tissue, and human glioma tissue. Flow Cyt: U-87 MG cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 3.80 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6774(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
WB | (1) |
1/10000 - 1/50000.
Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
特記事項 |
---|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
-
機能
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. -
組織特異性
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). -
配列類似性
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 6505 Human
- Omim: 133550 Human
- SwissProt: P43005 Human
- Unigene: 444915 Human
-
別名
- EAA3_HUMAN antibody
- EAAC 1 antibody
- EAAC1 antibody
see all
画像
-
All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution ((unpurified))
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and Diluting buffer : 5% NFDM/TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling EAAT3 with purified ab124802 at 1/1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?We are unsure how to define the extra bands.
-
Unpurified ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (16)
ab124802 は 16 報の論文で使用されています。
- Meira W et al. A Cystine-Cysteine Intercellular Shuttle Prevents Ferroptosis in xCTKO Pancreatic Ductal Adenocarcinoma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33801101
- Kinoshita C et al. Inhibition of miR-96-5p in the mouse brain increases glutathione levels by altering NOVA1 expression. Commun Biol 4:182 (2021). PubMed: 33568779
- Xiong J et al. SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential. EBioMedicine 72:103614 (2021). PubMed: 34628354
- Dabrowska-Bouta B et al. Early Postnatal Exposure to a Low Dose of Nanoparticulate Silver Induces Alterations in Glutamate Transporters in Brain of Immature Rats. Int J Mol Sci 21:N/A (2020). PubMed: 33256007
- Liu XX et al. Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis. J Exp Med 217:N/A (2020). PubMed: 31699822