リコンビナントRabMAb

Anti-EAAT2 抗体 [EPR19798] - BSA and Azide free (ab271970)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
  • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
  • Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19798] to EAAT2 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-EAAT2 antibody [EPR19798] - BSA and Azide free
    EAAT2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19798] to EAAT2 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Rat
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat striatum tissues. IHC-Fr: Mouse striatum tissue. IP: Mouse brain lysate.

  • 特記事項

    ab271970 is the carrier-free version of ab205248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19798

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Rat
IP
Mouse
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-Fr
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

  • 配列類似性

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

  • 翻訳後修飾

    Glycosylated.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EAA2_HUMAN antibody
      • EAAT2 antibody
      • Excitatory amino acid transporter 2 antibody
      • Excitotoxic amino acid transporter 2 antibody
      • Glial high affinity glutamate transporter antibody
      • GLT 1 antibody
      • GLT1 antibody
      • Glutamate aspartate transporter II antibody
      • Glutamate transporter 1 antibody
      • Glutamate/aspartate transporter II antibody
      • Slc1a2 antibody
      • Sodium dependent glutamate aspartate transporter 2 antibody
      • Sodium-dependent glutamate/aspartate transporter 2 antibody
      • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
      • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
      • Solute carrier family 1 member 2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on mouse kidney [PMID: 11038258].

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

      Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse striatum is observed [PMID 25391854]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

      Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
    • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854].  The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
    • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

      EAAT2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab205248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse brain lysate, 10μg (Input).

      Lane 2: ab205248 IP in Mouse brain lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab205248 in Mouse brain lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
    • Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
      Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271970 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271970 は論文での使用が確認できていません。

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