Anti-EAAT1 抗体 (ab416)
Key features and details
- Rabbit polyclonal to EAAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EAAT1 antibody
EAAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EAAT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
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特記事項
Glutamate-aspartate transporter is also known as GLAST.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab416の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/200 - 1/5000. Predicted molecular weight: 60 kDa.
|
| IHC-P | (3) |
1/50 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/200 - 1/5000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/50 - 1/500. |
ターゲット情報
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機能
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
組織特異性
Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. -
関連疾患
Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. -
配列類似性
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6507 Human
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 34134 Rat
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別名
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
画像
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Western blot - Anti-EAAT1 antibody (ab416)Anti-EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 150 kDa (possible dimer) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (90)
ab416 は 90 報の論文で使用されています。
- Limbad C et al. Astrocyte senescence promotes glutamate toxicity in cortical neurons. PLoS One 15:e0227887 (2020). PubMed: 31945125
- Zhang RS et al. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. Elife 9:N/A (2020). PubMed: 32202499
- Silva-Adaya D et al. Early Neurotoxic Effects of Inorganic Arsenic Modulate Cortical GSH Levels Associated With the Activation of the Nrf2 and NF?B Pathways, Expression of Amino Acid Transporters and NMDA Receptors and the Production of Hydrogen Sulfide. Front Cell Neurosci 14:17 (2020). PubMed: 32194376
- Liu XX et al. Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis. J Exp Med 217:N/A (2020). PubMed: 31699822
- Matott MP et al. Sustained Hypoxia Alters nTS Glutamatergic Signaling and Expression and Function of Excitatory Amino Acid Transporters. Neuroscience 430:131-140 (2020). PubMed: 32032667