Anti-E2F4 抗体 [EPR8259] (ab150360)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8259] to E2F4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-E2F4 antibody [EPR8259]
E2F4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8259] to E2F4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
Database link: Q16254 -
ポジティブ・コントロール
- WB: HEK293T, K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8259 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein. -
組織特異性
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the E2F/DP family. -
発生段階
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle. -
翻訳後修飾
Differentially phosphorylated in vivo. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1874 Human
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
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別名
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
画像
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All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : E2F4 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.
ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab150360 は 6 報の論文で使用されています。
- Zheng Q et al. Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. J Cancer 12:1792-1803 (2021). PubMed: 33613768
- Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
- Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
- Wang SA et al. USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy. Sci Rep 10:20870 (2020). PubMed: 33257797
- Dhanasekaran K et al. Unraveling the role of aurora A beyond centrosomes and spindle assembly: implications in muscle differentiation. FASEB J 33:219-230 (2019). PubMed: 29995440
- Li LL et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). PubMed: 24886710