Anti-E2 tag 抗体 [3F12] (ab978)
Key features and details
- Mouse monoclonal [3F12] to E2 tag
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-E2 tag antibody [3F12]
E2 tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3F12] to E2 tag -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Species independent -
エピトープ
GVSSTSSDFRDR(linear) -
特記事項
E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
3F12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
追加情報
IF(ICC): 1/500- 1/1000 (1hr,RT) (dilute in 1% BSA in PBS).
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein. -
別名
- E2 epitope tag antibody
- SSTSSDFRDR epitope tag antibody
- SSTSSDFRDR tag antibody
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)](https://www.abcam.co.jp/ps/datasheet/Images/0/ab978/ab977_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)Staining of transfected COS-7 cells with COX-2 specific antibody (A, B, D) and with E2Tag-specific antibody (3F12)(C).
A - pcDNA3.1 rabbit COX-2,
B - pCG-E2Tag-COX,
C - pCG-COX[E2Tag]
D – neg. control, carrier DNA alone
参考文献
ab978 は 7 報の論文で使用されています。
- Wu K et al. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell 18:755-67 (2007). PubMed: 17182846
- Auvinen E et al. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol 149:1745-59 (2004). PubMed: 15593417
- Ord D & Ord T Mouse NIPK interacts with ATF4 and affects its transcriptional activity. Exp Cell Res 286:308-20 (2003). PubMed: 12749859
- Koljak R et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
- Kaldalu N et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
- Kaldalu N et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926
