Anti-E2 tag 抗体 [3F12] (ab978)

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Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)

    Key features and details

    • Mouse monoclonal [3F12] to E2 tag
    • Suitable for: WB, IP, ICC/IF
    • Reacts with: Species independent
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-E2 tag antibody [3F12]
      E2 tag 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [3F12] to E2 tag

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WB, IP, ICC/IFmore details

    • 種交差性

      交差種: Species independent

    • エピトープ

      GVSSTSSDFRDR(linear)

    • 特記事項

      E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      3F12

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab978の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    IP
    ICC/IF
    特記事項
    追加情報
    IF(ICC): 1/500- 1/1000 (1hr,RT) (dilute in 1% BSA in PBS).
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    ターゲット情報

    • 関連性

      The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein.

    • 別名

      • E2 epitope tag antibody
      • SSTSSDFRDR epitope tag antibody
      • SSTSSDFRDR tag antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)
      Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (ab978)

      Staining of transfected COS-7 cells with COX-2 specific antibody (A, B, D) and with E2Tag-specific antibody (3F12)(C).

      A - pcDNA3.1 rabbit COX-2,

      B - pCG-E2Tag-COX,

      C - pCG-COX[E2Tag]

      D – neg. control, carrier DNA alone

    参考文献 (7)

    ab978 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab978 は 7 報の論文で使用されています。

    • Wu K  et al. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell 18:755-67 (2007). PubMed: 17182846
    • Auvinen E  et al. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol 149:1745-59 (2004). PubMed: 15593417
    • Ord D & Ord T Mouse NIPK interacts with ATF4 and affects its transcriptional activity. Exp Cell Res 286:308-20 (2003). PubMed: 12749859
    • Koljak R  et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
    • Kaldalu N  et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
    • Kaldalu N  et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
    • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926

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