Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast cells)
Specification
live yeast cells
Preparation
Cell harvesting/tissue preparation method: harvest cells then wash with 1XPBS
Sample buffer: 1XPBS
Sample buffer: 1XPBS
Fixation
No fixation
Permeabilization
No
Gating Strategy
E-tag expression
Other product details
Dilution
1/1000
Incubation time
15 minute(s) · Temperature: 4°C · Diluent: 1XPBS
Secondary antibody
Name
Non-Abcam antibody was used: PE-conjugated donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/1000
Additional data
Additional Notes
This is a very good antibody for E-tag surface expression on yeast.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 10 2012