Anti-E tag 抗体 (ab3397)
Key features and details
- Rabbit polyclonal to E tag
- Suitable for: ICC, ELISA, WB, ICC/IF, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-E tag antibody
E tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to E tag -
由来種
Rabbit -
アプリケーション
適用あり: ICC, ELISA, WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide: GAPVPYPDPLEPR conjugated to KLH.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3397の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA).
A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction.
Appropriate specificity controls were run.
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| WB |
1/1000 - 1/3000.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
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ICC
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run. |
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WB
1/1000 - 1/3000. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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関連性
E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR. -
別名
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
画像
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Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreview
ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab3397 は 7 報の論文で使用されています。
- Chekli Y et al. Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Sci Rep 10:15791 (2020). PubMed: 32978420
- Zhao L et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1a. Sci Rep 9:11861 (2019). PubMed: 31413262
- Mont S et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
- Chavanayarn C et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . PubMed: 22852068
- Leotoing L et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem 286:32277-88 (2011). IP . PubMed: 21784860
- Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
- Lampugnani MG et al. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174:593-604 (2006). PubMed: 16893970