製品名E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein
This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.
アプリケーション適用あり: WBmore details
製品の状態Lyophilised:After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times
保存方法Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
The cell lysate was prepared from E.coli (Tuner [De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag.
Our Abpromise guarantee covers the use of ab2431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. This E. coli (strain BL21)lysate contains a recombinant C-terminal 6x-His tagged protein of 17 kDa. The his-tagged protein is monomeric and will be detected under reducing and non-reducing conditions. Use 5-20 µg per lane of a mini-gel at 12-15 % SDS-PAGE. After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and than stored at -20°C. The sample can be reused several times.|
Western blot of 9 µg of E. coli BL21 lysate containing a 17kDa 6x-His tagged protein. Mouse monoclonal to His tag (4D11 - ab5000) is used at 1:1000.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab2431 は 1 報の論文で使用されています。
- Wright JC et al. Enhanced peptide identification by electron transfer dissociation using an improved mascot percolator. Mol Cell Proteomics : (2012). PubMed: 22493177