E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

製品の概要

  • 製品名

    E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
  • 特記事項

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
    T7 HSV
    C-Myc
    VSV-g
    Glu-Glu
    V5
    E-tag
    FLAG (DDDDK)
    S-tag
    HA
    6x His

    GST

    Tag sequences: T7 = CMASMTGGQQMG
    HSV = QPELAPEDPEDC
    CMYC = EQKLISEEDLC
    VSV-g = YTDIEMNRLGK
    GluGlu = EYMPME
    V5 = GKPIPNPLLGLDST
    E-tag = GAPVPYPDPLEPR
    FLAG = DYKDDDDK
    HA = YPYDVPDYAC
    6xHIS = HHHHHH

     

    Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

  • アプリケーション

    適用あり: WBmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

画像

  • All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl
  • This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 11 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.

参考文献

This product has been referenced in:

See all 3 Publications for this product

レビューと Q&A

1-3 of 3 Q&A

Answer

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très désolée pour le retard de cette réponse. J'espère que cette information vous est quand-même utile.

Aussi, est-ce que vous avez eu des bons résultats avec le nouveau tube de ab2431?

Encore une fois toutes mes excuses pour ce délais de réponse. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Question
Answer

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC HA = YPYDVPDYAC VSV-g = YTDIEMNRLGK GluGlu = EYMPME E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK V5 = TSTLPVDTTDWIEGETYK 6xHIS = HHHHHH I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録