製品の概要

  • 製品名
    Anti-E. coli O + E. coli K antibody
    E. coli O + E. coli K 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to E. coli O + E. coli K
  • 由来種
    Rabbit
  • 特異性
    Reactive with all O and K antigenic serotypes of E. coli.
  • アプリケーション
    適用あり: Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Whole cell preparation E. coli, all serotypes

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ELISA 1/2000.

ターゲット情報

  • 関連性
    Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
  • 別名
    • Capsular antigen antibody
    • Escherichia coli antibody
    • Escherichia coli K antibody
    • Escherichia coli O antibody
    • K antigen antibody
    • O antigen antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Encarnação JC  et al. Detecting ligand interactions in real time on living bacterial cells. Appl Microbiol Biotechnol 102:4193-4201 (2018). Read more (PubMed: 29550990) »
  • Templier V  et al. Biochips for Direct Detection and Identification of Bacteria in Blood Culture-Like Conditions. Sci Rep 7:9457 (2017). Read more (PubMed: 28842712) »

See all 2 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Escherichia coli Cell (Bacterial over night culture of E.coli HB101)
Specification
Bacterial over night culture of E.coli HB101
Preparation
Cell harvesting/tissue preparation method: washed with PPS
Sample buffer: PBS containing 10% FCS, 1% BSA
Fixation
PFA 0,4% after staining
Permeabilization
No
Gating Strategy
total events (FSC, SSC log)
Username

Herr Dr. Jonathan Jantsch

Verified customer

投稿 Feb 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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