Anti-E Cadherin 抗体 [HECD-1] - Intercellular Junction Marker (ab1416)
Key features and details
- Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker
- Suitable for: Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker
E Cadherin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Rat, Human
非交差種: Mouse -
免疫原
Breast tumor cell line MCF-7
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ポジティブ･コントロール
- WB: MDA-MB-468 and LNCaP whole cell lysates; MCF7 cell lysate. ICC/IF: Human renal tubular epithelial cells; Human nasal epithelial cells; NSCLC cells; Human renal proximal tubular cells; LNCaP cells. IHC-P: Breast cancer tissue; Human stomach tissue. Flow Cyt: MCF7 and A431 cells. IHC-Fr: Human skin tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% Proprietary component -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
HECD-1 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab1416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | 1/50. |
ターゲット情報
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機能
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. -
組織特異性
Non-neural epithelial tissues. -
関連疾患
Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. -
配列類似性
Contains 5 cadherin domains. -
翻訳後修飾
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. -
細胞内局在
Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 999 Human
- Entrez Gene: 83502 Rat
- Omim: 192090 Human
- SwissProt: P12830 Human
- SwissProt: Q9R0T4 Rat
- Unigene: 461086 Human
- Unigene: 1303 Rat
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別名
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
see all
画像
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![Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-297427-ab141661724.jpg)
Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr. Armen Petrosyan.Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution + LNCaP (human prostate cancer cell line) whole cell lyate
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![Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416_2.jpg)
Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr Alban GaultierAll lanes : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/1000 dilution
All lanes : MDA-MB-468 cells whole cell lysate
Lysates/proteins at 80 µg/ml per lane.
Secondary
All lanes : HRP-conjugated polyclonal donkey anti-mouse IgG
Performed under reducing conditions.
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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![Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-250515-ab1416wb.jpg)
Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)Lanes 1-2 : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution
Lanes 3-4 : Anti-GAPDH at 1 µg/ml
Lanes 1 & 3 : MDA-MB-231 cell lysate
Lanes 2 & 4 : MCF-7 cell lysateBlocking buffer: 3% milk
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![Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-7-ab1416FC.jpg)
Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)Overlay histogram showing MCF7 cells stained with ab1416 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1416, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/E-Cadherin-Primary-antibodies-ab1416-86.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Michael Könneckeab1416 staining E Cadherin in Human nasal epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed paraformaldehyde and permeabilized with Triton X-100 0.1%. Samples were incubated with primary antibody (1/50) for 18 hours at 4°C. A Cy2®-conjugated Horse anti-mouse monoclonal(1/100) was used as the secondary antibody. -
![Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-297431-ab141637127.jpg)
Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is ccourtesy of anonymous Abreview.Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling E Cadherin with ab1416 at 1/100 dilution (2) compared with an isotype control (1).
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![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-262218-ab1416ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)Image courtesy of an anonymous Abreviewab1416 staining E Cadherin in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton in PBS and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal was used as the secondary antibody (1/500).
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![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/E-Cadherin-Primary-antibodies-ab1416-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an abreview submitted by Robert Jenkins.ab1416 staining E Cadherin in Human Renal Proximal Tubular cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton ×100 and blocking with 1% BSA was done for 30 minutes at 210C. Samples were incubated with primary antibody (1/50: in 0.1 % BSA in PBS) for 24 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-297425-ab141661726.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr. Armen Petrosyan.Formaldehyde-fixed, 0.2% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells stained for E Cadherin (green) using ab1416 at 1/50 dilution in ICC/IF. Blue: Hoechst 33342 staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)](https://www.abcam.co.jp/ps/products/1/ab1416/Images/ab1416-308613-anti-e-cadherin-antibody-hecd-1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)Immunohistochemistry of breast carcinoma staining E Cadherin with ab1416 at 5μg/ml
プロトコール
参考文献
ab1416 は 361 報の論文で使用されています。
- Liang L et al. Oxymatrine reverses 5-fluorouracil resistance by inhibition of colon cancer cell epithelial-mesenchymal transition and NF-?B signaling in vitro. Oncol Lett 19:519-526 (2020). PubMed: 31897166
- Sun X et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
- Gong J et al. Reprogramming of lipid metabolism in cancer-associated fibroblasts potentiates migration of colorectal cancer cells. Cell Death Dis 11:267 (2020). PubMed: 32327627
- Dong C et al. CircSMARCA5 Facilitates the Progression of Prostate Cancer Through miR-432/PDCD10 Axis. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32407167
- Zhou C et al. lncRNA HOXC-AS1 promotes gastric cancer via binding eIF4AIII by activating Wnt/ß-catenin signaling. J Gene Med N/A:e3202 (2020). PubMed: 32307743
