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  1. Link

    e-cadherin-antibody-hecd-1-intercellular-junction-marker-ab1416.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-E Cadherin 抗体 [HECD-1] - Intercellular Junction Marker (ab1416)

  • Datasheet
  • SDS
Reviews (42)Q&A (22)References (573)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

Key features and details

  • Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker
    E Cadherin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, Flow Cyt, WBmore details
  • 種交差性

    交差種: Human
    非交差種: Mouse
  • 免疫原

    This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: MDA-MB-468 and LNCaP whole cell lysates; MCF7 cell lysate. IHC-P: Breast cancer tissue; Human stomach tissue. Flow Cyt: MCF7 and A431 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% Proprietary component
  • Concentration information loading...
  • 精製度

    Ascites
  • 特記事項(精製)

    This product is ascites-derived.
  • ポリ/モノ

    モノクローナル
  • クローン名

    HECD-1
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development
    • Neuroscience
    • Development

関連製品

  • Alternative Versions

    • Anti-E Cadherin antibody [4A2] (ab231303)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human E Cadherin protein (ab235682)
  • Related Products

    • Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)
    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1416の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (8)
Use at an assay dependent concentration.
Flow Cyt (1)
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (15)
1/50.
特記事項
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/50.

ターゲット情報

  • 機能

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • 組織特異性

    Non-neural epithelial tissues.
  • 関連疾患

    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • 配列類似性

    Contains 5 cadherin domains.
  • 翻訳後修飾

    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • 細胞内局在

    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Target information above from: UniProt accession P12830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 999 Human
    • Omim: 192090 Human
    • SwissProt: P12830 Human
    • Unigene: 461086 Human
    • 別名

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Immunohistochemical analysis of human breast cancer tissue labeling E Cadherin with ab1416.

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr. Armen Petrosyan.
      Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution + LNCaP (human prostate cancer cell line) whole cell lyate

      See Abreview

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr Alban Gaultier
      All lanes : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/1000 dilution

      All lanes : MDA-MB-468 cells whole cell lysate

      Lysates/proteins at 80 µg/ml per lane.

      Secondary
      All lanes : HRP-conjugated polyclonal donkey anti-mouse IgG

      Performed under reducing conditions.

      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Lanes 1-2 : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution
      Lanes 3-4 : Anti-GAPDH at 1 µg/ml

      Lanes 1 & 3 : MDA-MB-231 cell lysate
      Lanes 2 & 4 : MCF-7 cell lysate


      Blocking buffer: 3% milk

    • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Overlay histogram showing MCF7 cells stained with ab1416 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1416, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is ccourtesy of anonymous Abreview.

      Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling E Cadherin with ab1416 at 1/100 dilution (2) compared with an isotype control (1).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is a courtesy of Anonymous Abreview

      ab1416 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Immunohistochemistry of human breast carcinoma staining E Cadherin with ab1416 at 5μg/ml

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (573)

    ab1416 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1416 は 573 報の論文で使用されています。

    • Yan Y  et al. The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis. Cell Death Discov 8:81 (2022). WB ; Human . PubMed: 35210429
    • Chen S  et al. Cancer-associated fibroblast-induced M2-polarized macrophages promote hepatocellular carcinoma progression via the plasminogen activator inhibitor-1 pathway. Int J Oncol 59:N/A (2021). PubMed: 34195849
    • Zhang Y  et al. Long non-coding RNA CYTOR regulates proliferation and metastasis of colon cancer cells through regulating miRNA-105/PTEN axis. Int J Clin Exp Pathol 14:434-443 (2021). PubMed: 33936365
    • Fu Q  et al. CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinoma. J Transl Med 19:210 (2021). PubMed: 34001131
    • You J & Wang X Circ_HIPK3 Knockdown Inhibits Cell Proliferation, Migration and Invasion of Cholangiocarcinoma Partly via Mediating the miR-148a-3p/ULK1 Pathway. Cancer Manag Res 13:3827-3839 (2021). PubMed: 34007215
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 64 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (Fetal Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Fetal Heart
    Blocking step
    EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Dr. Juliana Shimara Pires Ferrão

    Verified customer

    投稿 Nov 26 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2020

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (liver)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (6%)
    Loading amount
    20 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: PH=6 ,citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 18 2020

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human skin sample)
    Gel Running Conditions
    Reduced Denaturing (8% SDS page gel)
    Loading amount
    30 µg
    Treatment
    30Gy irradiation for 24hr
    Specification
    Human skin sample
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (mammary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate ph 6,0
    Permeabilization
    No
    Specification
    mammary gland
    Blocking step
    Background Block (CELL MARQUE) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Prof. ALESSANDRA MEDEIROS

    Verified customer

    投稿 Oct 15 2018

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    20 µg
    Specification
    Mice hepatocytes - plasma membrane fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Apr 25 2018

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (N/A)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    cleavage assay
    Specification
    N/A
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP - prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    LNCaP - prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    DR. Armen Petrosyan

    Verified customer

    投稿 Sep 26 2017

    Question

    Thanks for your kindly reply, this customer already used a positive control (Huh7, is ab1416 abreview suggested), and the results seem no problem. Furthermore, this customer indicated she also used the ab53033 and conducted wb assay with the same condition as this time, and the results show strong signal, I attached the image in this letter, could you please help this customer to solve the problem?   Thanks for your kindly assistance.   Best regards

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    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    Thank you for sending the image. It may well be that the vial you have received was a bad vial which is why the antibody is not detecting the target although it is very strictly qc tested. If you are interested in trying the same antibody I can send you a free of charge replacement vial. A credit note or refund can also be provided if you are happy with the result of ab53033. I would also like to encourage you to submit an Abreview for ab53033; you will be awarded Abpoints that can be exchanged with Amazon vouchers. Let me know how you would like to proceed.

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    Abcam Scientific Support

    Answered on Nov 24 2011

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