Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Permeabilization
Yes - 0.05%Tween-20
Specification
Liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/20
Secondary antibody
Name
Abcam antibody:Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Alexa Fluor® 488 Green immunofluorescence staining of paraffin embedded rabbit liver with purified ab11512 Anti-E Cadherin at a working dilution of 1 in 20. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining and by using the 555nm excitation filter. Alexa Fluor 647 secondary offers better resolution and it lacks autofluorescence.
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投稿 Jun 25 2020