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  1. Link

    dynll1pin-antibody-ep1660y-ab51603.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-DYNLL1/PIN 抗体 [EP1660Y] (ab51603)

  • Datasheet
Reviews (7)Q&A (2)References (23)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Flow Cytometry - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
  • Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1660Y] to DYNLL1/PIN
  • Suitable for: Flow Cyt, WB, IHC-P, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human DYNLL1/PIN protein (ab101204)
ペプチド
Product image
V5 tag peptide (ab15829)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-DYNLL1/PIN antibody [EP1660Y]
    DYNLL1/PIN 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP1660Y] to DYNLL1/PIN
  • 由来種

    Rabbit
  • 特異性

    ab51603 recognizes DLC8. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-P, IP, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human DYNLL1/PIN aa 1-100 (N terminal).

  • エピトープ

    The epitope for this antibody is on the N-terminus, AA2-14.
  • ポジティブ・コントロール

    • WB: HeLa cell lysate; Mouse testis tissue lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells.
  • 特記事項

    Previously labelled as DYNLL1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EP1660Y
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-DYNLL1/PIN antibody [EP1660Y] (ab203988)
    • Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (ab232343)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human DYNLL1 (PIN) knockout HeLa cell line (ab265265)
  • KO cell lysates

    • Human DYNLL1 (PIN) knockout HeLa cell lysate (ab257414)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human DYNLL1/PIN protein (ab101204)

アプリケーション

Our Abpromise guarantee covers the use of ab51603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/2300.
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

IP 1/30.

For unpurified use at 1/100.

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
    Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
    Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
    Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
  • 組織特異性

    Ubiquitous.
  • 配列類似性

    Belongs to the dynein light chain family.
  • 翻訳後修飾

    Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.
  • 細胞内局在

    Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.
  • Target information above from: UniProt accession P63167 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 31405 Drosophila melanogaster
    • Entrez Gene: 8655 Human
    • Entrez Gene: 56455 Mouse
    • Entrez Gene: 58945 Rat
    • Omim: 601562 Human
    • SwissProt: Q24117 Drosophila melanogaster
    • SwissProt: P63167 Human
    • SwissProt: P63168 Mouse
    • SwissProt: P63170 Rat
    • Unigene: 5892 Drosophila melanogaster
    • Unigene: 5120 Human
    • Unigene: 256858 Mouse
    • Unigene: 35769 Rat
    see all
  • 別名

    • 8 kDa dynein light chain antibody
    • 8kDLC antibody
    • Cytoplasmic dynein light polypeptide antibody
    • DLC1 antibody
    • DLC8 antibody
    • DNCL1 antibody
    • DNCLC1 antibody
    • DYL1_HUMAN antibody
    • Dynein , cytoplasmic, light chain 1 antibody
    • Dynein light chain 1 cytoplasmic antibody
    • Dynein light chain 1, cytoplasmic antibody
    • Dynein light chain LC8 type 1 antibody
    • Dynein light chain LC8-type 1 antibody
    • Dynein, cytoplasmic, light polypeptide 1 antibody
    • Dynein, light chain, LC8-type 1 antibody
    • DYNLL1 antibody
    • HDLC1 antibody
    • LC8 antibody
    • LC8a antibody
    • MGC126137 antibody
    • MGC126138 antibody
    • MGC72986 antibody
    • PIN antibody
    • Protein inhibitor of neuronal nitric oxide synthase antibody
    • Protein inhibitor of neuronal NOS antibody
    see all

画像

  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    All lanes : Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : DYNLL1 knockout HeLa cell lysate
    Lane 3 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab51603 observed at 10 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab51603 Anti-DYNLL1/PIN antibody [EP1660Y] was shown to specifically react with DYNLL1/PIN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265265 (knockout cell lysate ab257414) was used. Wild-type and DYNLL1/PIN knockout samples were subjected to SDS-PAGE. ab51603 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    All lanes : Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603) at 1/10000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse testis lysates
    Lane 3 : Rat testis lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

    ab51603 (purified) at 1:30 dilution (2ug) immunoprecipitating DYNLL1/PIN in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab51603 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51603 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DYNLL1/PIN with Purified ab51603 at 1:100 dilution (6.7μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling DYNLL1/PIN with Purified ab51603 at 1:500 dilution (1.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)This image is courtesy of an abreview submitted by Dr. Jörg Heierhorst
    All lanes : Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603) at 1/5000 dilution (unpurified)

    Lane 1 : Primary mouse Mb1-Cre control Eµ-Myc B cell lymphoma (lysate of whole lymphnode)
    Lane 2 : Primary mouse Mb1-Cre DYNLL1/PIN-conditional knockout Eµ-Myc B cell lymphoma (lysate of whole lymphnode)

    Secondary
    All lanes : HRP conjugated polyclonal goat IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 10 kDa
    Observed band size: 10 kDa


    Exposure time: 10 minutes


    Lymphnodes were dissociated in PBS 2% FBS. Cell suspensions filtered through 70 µm and 40 µm cell strainers, and 300 x g pellets were lysed in modified RIPA buffer (150 mM NaCl, 20 mM Tris pH7.4, 1 mM EDTA, 1 mM EGTA, 10 mM NaF, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 x protein inhibitor cocktail (Sigma)).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

    Immunohistochemical staining of paraffin embedded human liver using unpurified ab51603 (1/100).

  • Flow Cytometry - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Flow Cytometry - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DYNLL1/PIN (red) with purified ab51603 at a 1/2300 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Western blot - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603) at 1/10000 dilution (unpurified) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)This image is courtesy of an anonymous Abreview

    Unpurified ab51603 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.

    See Abreview

  • Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)Image from Schmidt JC et al, J Cell Biol. 2010 Oct 18;191(2):269-80. Epub 2010 Oct 11, Fig 2. DOI 10.1083/jcb.201006129

    Unpurified ab51603 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.

  • Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)
    Anti-DYNLL1/PIN antibody [EP1660Y] (ab51603)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (23)

    ab51603 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab51603 は 23 報の論文で使用されています。

    • Kiuchi Z  et al. GLCCI1 is a novel protector against glucocorticoid-induced apoptosis in T cells. FASEB J 33:7387-7402 (2019). PubMed: 30860871
    • King A  et al. Dynll1 is essential for development and promotes endochondral bone formation by regulating intraflagellar Dynein function in primary cilia. Hum Mol Genet N/A:N/A (2019). PubMed: 31009951
    • Liu K  et al. PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Dev Cell 50:509-524.e10 (2019). PubMed: 31327739
    • Ren G  et al. Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFß. Sci Rep 9:6688 (2019). PubMed: 31040372
    • Goldman CH  et al. The Egalitarian binding partners Dynein light chain and Bicaudal-D act sequentially to link mRNA to the Dynein motor. Development 146:N/A (2019). PubMed: 31391195
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 24 2017

    Western blot abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (B cell lymphoma lymphnodes)
    Gel Running Conditions
    Reduced Denaturing (15% midigel)
    Loading amount
    12 µg
    Specification
    B cell lymphoma lymphnodes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Jörg Heierhorst

    Verified customer

    投稿 Apr 13 2016

    Western blot abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (15% GEL)
    Loading amount
    30 µg
    Specification
    mouse Embryonic Fibroblasts
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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    投稿 May 31 2014

    Western blot abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (H23)
    Gel Running Conditions
    Reduced Denaturing (15% GEL)
    Loading amount
    20 µg
    Specification
    H23
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

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    投稿 Dec 13 2013

    Question

    Bonjour, J'aimerais tester votre anticorps anti DyNLL1 EP1660Y ab51603 sur la Drosophile (IF et/ou IP), serait il possible d'en obtenir un petit aliquot à fin de le tester avant d'acheter le conditionnement 100ul. Merci de votre aide.

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    Abcam community

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    Asked on Nov 29 2012

    Answer

    Code de remise : xxxxxx
    Date d’expiration : 30/11/12

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab51603 chez la drosophile. Le code de réduction correspondant est xxxxxxxxx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire.

    Vous devez dans un premier temps soumettre une Abreview avec vos résultatschez la drosophile en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab51603.

    Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

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    Abcam Scientific Support

    Answered on Nov 29 2012

    Question

    Bonjour, J'aimerais tester votre anticorps anti DyNLL1 EP1660Y ab51603 sur la Drosophile (IF et/ou IP), serait il possible d'en obtenir un petit aliquot à fin de le tester avant d'acheter le conditionnement 100ul. Merci de votre aide.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2012

    Answer

    Merci de votre intérêt pour ab51603. A ma connaissance, cet anticorps n’a pas encore été testé chez la Drosophile et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.

    J’ai fait un alignement entre la protéine chez l’humain et la drosophile. Il y a une homologie totale de 100% entre l’immunogen des deux protéines, donc cet anticorps à de grandes chances de fonctionner chez la drosophile.

    Si vous souhaitez tester ab51603 chez la Drosophile, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab51603 pourra être utilisable lors d’une prochaine commande d’anticorps primaire.

    Etapes à suivre :

    1. Nous confirmer que vous souhaiteriez tester ab51603 chez la Drosophile afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab51603

    2. Acheter ab51603 par téléphone, fax ou internet (www.abcam.com)

    3. Le tester chez la Drosophile

    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre produit.
    Même si ab51603 est démontré ne pas fonctionner chez la Drosophile, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.

    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

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    Abcam Scientific Support

    Answered on Nov 29 2012

    Western blot abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Recombinant protein (Recombinant protein cloned into EcoR1)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine Gel)
    Loading amount
    0.5 µg
    Specification
    Recombinant protein cloned into EcoR1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 07 2010

    Western blot abreview for Anti-DYNLL1 / PIN antibody [EP1660Y]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12 % gradient gel, Novex)
    Loading amount
    20 µg
    Specification
    HeLa
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 30 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DYNLL1/PIN antibody [EP1660Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kidney)
    Permeabilization
    Yes - 0.1 % Triton
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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