製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DUSP8
  • 由来種
    Goat
  • 特異性
    This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide: AGDRLPRKVMDAK, corresponding to N terminal amino acids 2-14 of Human DUSP8.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human DUSP8 peptide (ab22818).

Approx. 60-65kDa bands seen in Human Heart lysate.

ターゲット情報

  • 機能
    This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.
  • 組織特異性
    Abundant in brain, heart and skeletal muscle.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C11orf81 antibody
    • Chromosome 11 open reading frame 81 antibody
    • Deubiquitinating enzyme 8 antibody
    • Dual Specificity Phosphatase 8 antibody
    • Dual specificity phosphatase M3/6 antibody
    • Dual specificity protein phosphatase 8 antibody
    • Dual specificity protein phosphatase hVH 5 antibody
    • Dual specificity protein phosphatase hVH-5 antibody
    • DUS8 antibody
    • DUS8_HUMAN antibody
    • DUSP8 antibody
    • EC 3.1.3.16 antibody
    • EC 3.1.3.48 antibody
    • FLJ42476 antibody
    • FLJ42958 antibody
    • H1 phosphatase vaccinia virus homolog antibody
    • H1 phosphatase, vaccinia virus, homolog of antibody
    • HB5 antibody
    • hUBPy antibody
    • HVH5 antibody
    • HVH8 antibody
    • M3/6, mouse, homolog of antibody
    • Neuronal tyrosine threonine phosphatase 1 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Ubiquitin carboxyl terminal hydrolase 8 antibody
    • Ubiquitin specific processing protease 8 antibody
    • Ubiquitin thiolesterase 8 antibody
    • VH5 antibody
    see all

画像

  • Ab1363 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Ab1363 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

プロトコール

参考文献

ab1363 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. I am sorry to hear you are having a problem with ab1363. I would like to suggest the following modifications to your protocol: One change I can suggest in your protocol is to do 5 x 5 minutes washes for primary and se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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