Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)

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Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)

    製品の概要

    • 製品名

      Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control
      Mouse アイソタイプ・コントロール 製品一覧

    • 標識

      Dual color: FITC + PE

    • アプリケーション

      適用あり: Flow Cytmore details

    • 特記事項

      This product is a mixture of the same clone separately bearing both FITC and PE labels. This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.


      This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.1% Sodium azide
      Constituent: 0.5% BSA
    • Concentration information loading...

    • アイソタイプ・コントロール 備考

      This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

    • ポリ/モノ

      モノクローナル

    • クローン名

      HybIgG1

    • ミエローマ

      unknown

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    • 別名

      • Mouse Isotype Control

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1285の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use at an assay dependent concentration.

    This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells.
    特記事項
    Flow Cyt
    Use at an assay dependent concentration.

    This antibody control duo is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC + PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. Scatter gates are set on the lymphocyte fraction. Proper electronic compensation and filter selections are necessary for three-color analysis. 10µl ofIgG1 (FITC + PE) is sufficient for labelling of 1x106 cells.

    画像

    • Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
      Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
      ICC/IF image of ab2185 stained MCF7 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献 (0)

    ab1285 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1285 は論文での使用が確認できていません。

    レビューと Q&A

    Q&A
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