Anti-DTYMK 抗体 [EPR10163] (ab154867)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10163] to DTYMK
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DTYMK antibody [EPR10163]
DTYMK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10163] to DTYMK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Raji, K562, HeLa cell lysate; Human thyroid gland carcinoma tissue; HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10163 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154867の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/1000. |
ターゲット情報
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機能
Catalyzes the conversion of dTMP to dTDP. -
パスウェイ
Pyrimidine metabolism; dTTP biosynthesis. -
配列類似性
Belongs to the thymidylate kinase family. - Information by UniProt
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参照データベース
- Entrez Gene: 1841 Human
- Omim: 188345 Human
- SwissProt: P23919 Human
- Unigene: 471873 Human
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別名
- CDC8 antibody
- Deoxythymidylate kinase (thymidylate kinase) antibody
- deoxythymidylate kinase antibody
see all
画像
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All lanes : Anti-DTYMK antibody [EPR10163] (ab154867) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling DTYMK with ab137875 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling DTYMK with ab154867 at 1/100.
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Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DTYMK with purified ab154867 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154867 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154867 は 2 報の論文で使用されています。
- Zhou T et al. DTYMK promote hepatocellular carcinoma proliferation by regulating cell cycle. Cell Cycle 20:1681-1691 (2021). PubMed: 34369850
- He G et al. MiR-148b-3p Regulates the Expression of DTYMK to Drive Hepatocellular Carcinoma Cell Proliferation and Metastasis. Front Oncol 11:625566 (2021). PubMed: 35004265