Anti-Dscam 抗体 [EPR22435-247] - BSA and Azide free (Detector) (ab270327)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22435-247] to Dscam - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Anti-Dscam antibody [EPR22435-247] - BSA and Azide free (Detector)
Dscam 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22435-247] to Dscam - BSA and Azide free (Detector) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab270327 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify DSCAM. The recommended pair for sandwich ELISA is:
Capture: ab270326, DSCAM Capture Antibody (unconjugated)
Detector: ab270327, DSCAM Detector Antibody (unconjugated)
The reference range value is 203.125 - 13000 pg/ml.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22435-247 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270327の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
ターゲット情報
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機能
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. -
組織特異性
Primarily expressed in brain. -
配列類似性
Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC. -
翻訳後修飾
Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. -
細胞内局在
Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons. - Information by UniProt
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参照データベース
- Entrez Gene: 1826 Human
- Entrez Gene: 13508 Mouse
- Entrez Gene: 171119 Rat
- Omim: 602523 Human
- SwissProt: O60469 Human
- SwissProt: Q9ERC8 Mouse
- SwissProt: Q8VHZ8 Rat
- Unigene: 397800 Human
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別名
- CHD2 42 antibody
- CHD2 52 antibody
- CHD2 antibody
see all
画像
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Representative standard curve from corresponding SimpleStep ELISA® Kit (ab269543).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab270327 は論文での使用が確認できていません。