Anti-Drosophila FMR1 抗体 [6A15] (ab10299)
Key features and details
- Mouse monoclonal [6A15] to Drosophila FMR1
- Suitable for: ELISA, ICC/IF, IP, WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
製品の概要
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製品名
Anti-Drosophila FMR1 antibody [6A15] -
製品の詳細
Mouse monoclonal [6A15] to Drosophila FMR1 -
由来種
Mouse -
アプリケーション
適用あり: ELISA, ICC/IF, IP, WBmore details -
種交差性
交差種: Drosophila melanogaster
非交差種: Human -
免疫原
Fusion protein corresponding to Drosophila melanogaster Drosophila FMR1. His-dFMR1 fusion protein (Drosophilia melanogaster) (C-terminal 580aa).
Database link: Q9NFU0 -
特記事項
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Protein A purified from tissue culture supernatant. -
一次抗体 備考
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway. -
ポリ/モノ
モノクローナル -
クローン名
6A15 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
|
| IP | (1) |
Use at an assay dependent concentration.
See Abreview. |
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. See Abreview. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Drosophila FMR1 is a RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator of specific mRNAs. Represses translation of futsch to regulate microtubule-dependent synaptic growth and function. Part of the RNA interference (RNAi)-related apparatus; double-stranded RNA induces potent and specific gene silencing. Regulates photoreceptor structure and neurotransmission in the eye. Required for stability of the central pair of microtubules in the spermatid axoneme -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 37528 Drosophila melanogaster
- SwissProt: Q9NFU0 Drosophila melanogaster
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別名
- dFMR antibody
- dFMR1 antibody
- dFXR antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab10299 は 14 報の論文で使用されています。
- Sears JC & Broadie K FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory Circuitry. Cell Rep 33:108266 (2020). PubMed: 33053340
- Lee SK et al. Topoisomerase 3ß interacts with RNAi machinery to promote heterochromatin formation and transcriptional silencing in Drosophila. Nat Commun 9:4946 (2018). PubMed: 30470739
- Bienkowski RS et al. The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons. Cell Rep 20:1372-1384 (2017). IP ; Drosophila melanogaster . PubMed: 28793261
- Monyak RE et al. Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. Mol Psychiatry 22:1140-1148 (2017). PubMed: 27090306
- Ahmad M et al. RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals. Nucleic Acids Res 44:6335-49 (2016). WB ; Drosophila melanogaster . PubMed: 27257063