Anti-DR5 抗体 (ab1675)
Key features and details
- Goat polyclonal to DR5
- Suitable for: IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DR5 antibody
DR5 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DR5 -
由来種
Goat -
特異性
<0.2% cross-reactivity to TRAIL R3 peptide of the same region. Not likely to bind TRAIL R1 due to low sequence homology in this region. Antibody binds to a similar size protein from mouse spleen on Westerns. -
アプリケーション
適用あり: IHC-P, Flow Cyt, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
APQQKRSSPSEGLCPPGHHISEDGRDC
, corresponding to amino acids 68-94 of Human TRAIL Receptor 2. -
特記事項
TRAIL R2 = "TNF Related Apoptosis Inducing Ligand Receptor 2"
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab1675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/300. | |
| Flow Cyt | Use 1µl for 106 cells. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| ICC | 1/300. |
ターゲット情報
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機能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
組織特異性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
関連疾患
Squamous cell carcinoma of the head and neck -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8795 Human
- Omim: 603612 Human
- SwissProt: O14763 Human
- Unigene: 521456 Human
- Unigene: 661668 Human
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別名
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab1675)ab1675, at a dilution of 1/300, staining DR5 in human spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
プロトコール
参考文献 (3)
ab1675 は 3 報の論文で使用されています。
- Wagner J et al. Dose intensification of TRAIL-inducing ONC201 inhibits metastasis and promotes intratumoral NK cell recruitment. J Clin Invest 128:2325-2338 (2018). PubMed: 29533922
- Zhang S et al. A novel chalcone derivative S17 induces apoptosis through ROS dependent DR5 up-regulation in gastric cancer cells. Sci Rep 7:9873 (2017). PubMed: 28852176
- Wagner J et al. Preclinical evaluation of the imipridone family, analogs of clinical stage anti-cancer small molecule ONC201, reveals potent anti-cancer effects of ONC212. Cell Cycle 16:1790-1799 (2017). PubMed: 28489985
