Anti-DR5 抗体 (ab16329)
Key features and details
- Rabbit polyclonal to DR5
- Suitable for: ICC, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DR5 antibody
DR5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DR5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide:
CVPEQEMEVQEPAEPTG
, corresponding to amino acids 255-270 of Rat DR5. -
ポジティブ･コントロール
- WB: HeLa, PC12, A549 and HEL 92.1.7 cell lysate. ICC: HeLa and HCT 116 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16329の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 2 - 3 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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| 特記事項 |
|---|
|
ICC
Use a concentration of 2 - 3 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
組織特異性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
関連疾患
Squamous cell carcinoma of the head and neck -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8795 Human
- Entrez Gene: 364420 Rat
- Omim: 603612 Human
- SwissProt: O14763 Human
- Unigene: 521456 Human
- Unigene: 661668 Human
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別名
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
画像
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Western blot - Anti-DR5 antibody (ab16329)All lanes : Anti-DR5 antibody (ab16329) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC12 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunocytochemistry - Anti-DR5 antibody (ab16329)Immunocytochemistry analysis of DR5 was done on 70% confluent log phase HeLa cell. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab16329 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-DR5 antibody (ab16329)All lanes : Anti-DR5 antibody (ab16329) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HEL 92.1.7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/2500 dilution
Predicted band size: 58 kDaDetected by chemiluminescence
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Immunocytochemistry - Anti-DR5 antibody (ab16329)Immunocytochemistry analysis of DR5 (TRAIL-R2) was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab16329 at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green) in HCT 116 treated cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin 1/300. Panel d represents the merged image of HCT116 treated cells, which shows higher expression for TRAIL-R2 protein showing localization in nucleus, cytoplasm and membrane. Panel e represents the merged image of untreated HCT 116 cells, that shows lower or no expression for TRAIL-R2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab16329 は 3 報の論文で使用されています。
- Wu LS et al. LPS Enhances the Chemosensitivity of Oxaliplatin in HT29 Cells via GSDMD-Mediated Pyroptosis. Cancer Manag Res 12:10397-10409 (2020). PubMed: 33116894
- Wu LS et al. TRAIL inhibits platelet-induced colorectal cancer cell invasion. J Int Med Res 47:962-972 (2019). PubMed: 30621488
- Catlin NR et al. The Stage-specific Testicular Germ Cell Apoptotic Response to Low-dose Radiation and 2,5-hexanedione Combined Exposure. II: qRT-PCR Array Analysis Reveals Dose Dependent Adaptive Alterations in the Apoptotic Pathway. Toxicol Pathol N/A:N/A (2014). Rat . PubMed: 24670816
