Key features and details
- Rabbit polyclonal to DR4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab8415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 57 kDa.|
機能Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
組織特異性Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
配列類似性Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
- Information by UniProt
- Apo2 antibody
- CD261 antibody
- Cytotoxic TRAIL receptor antibody
All lanes : Anti-DR4 antibody (ab8415)
Lane 1 : H: HeLa
Lane 2 : K: K562
Lane 3 : J: Jurkat whole cell lysate
Observed band size: 57 kDa why is the actual band size different from the predicted?
Western blot analysis of DR4 at 1:500 dilution.
Immunocytochemistry of DR4 in Hela cells with ab8415 at 2 μg/mL.
ab8415 staining DR4 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes at 16°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in 1.5% Normal horse serum) for 2 hours at 16°C. An undiluted Biotin-conjugated Human pan-specific polyclonal was used as the secondary antibody.
ab8415 は 2 報の論文で使用されています。
- Spano C et al. Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer. Sci Rep 9:1788 (2019). PubMed: 30742129
- Köhler B et al. Bid participates in genotoxic drug-induced apoptosis of HeLa cells and is essential for death receptor ligands' apoptotic and synergistic effects. PLoS ONE 3:e2844 (2008). WB ; Human . PubMed: 18665234