Anti-Dopamine Receptor D1 抗体 (ab40653)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Dopamine Receptor D1 antibody
Dopamine Receptor D1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dopamine Receptor D1 -
由来種
Rabbit -
特異性
This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Dopamine Receptor D1 aa 1-100 (Cysteine residue).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40653の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
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IHC-P |
1/500.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
IHC-P
1/500. |
ターゲット情報
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機能
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
組織特異性
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
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参照データベース
- Entrez Gene: 281125 Cow
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: Q95136 Cow
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
see all -
別名
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
画像
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ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (15)
ab40653 は 15 報の論文で使用されています。
- Gao S et al. Striatal D1 Dopamine Neuronal Population Dynamics in a Rat Model of Levodopa-Induced Dyskinesia. Front Aging Neurosci 14:783893 (2022). PubMed: 35185524
- Agustí A et al. Bacteroides uniformis CECT 7771 Modulates the Brain Reward Response to Reduce Binge Eating and Anxiety-Like Behavior in Rat. Mol Neurobiol 58:4959-4979 (2021). PubMed: 34228269
- Felippe RM et al. Experimental Social Stress: Dopaminergic Receptors, Oxidative Stress, and c-Fos Protein Are Involved in Highly Aggressive Behavior. Front Cell Neurosci 15:696834 (2021). PubMed: 34489642
- Eom TY et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun 11:912 (2020). PubMed: 32060266
- Williams MT et al. Whole brain proton irradiation in adult Sprague Dawley rats produces dose dependent and non-dependent cognitive, behavioral, and dopaminergic effects. Sci Rep 10:21584 (2020). PubMed: 33299021