Anti-Dopamine Receptor D1 抗体 (ab20066)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Dopamine Receptor D1 antibody
Dopamine Receptor D1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dopamine Receptor D1 -
由来種
Rabbit -
特異性
Stains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0% -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide:
MDGTGLVVERDFS
, corresponding to amino acids 9-21 of Human Dopamine Receptor D1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20066の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
-
機能
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
組織特異性
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
-
参照データベース
- Entrez Gene: 1812 Human
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: P18901 Rat
- Unigene: 2624 Human
- Unigene: 24039 Rat
-
別名
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
画像
-
Western blot - Anti-Dopamine Receptor D1 antibody (ab20066)All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Forebrain
Lane 3 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (40)
ab20066 は 40 報の論文で使用されています。
- Zarrin P et al. Liposomal Form of L-Dopa and SH-Sy5y Cell-Derived Exosomes Modulate the Tyrosine Hydroxylase/Dopamine Receptor D2 Signaling Pathway in Parkinson's Rat Models. J Mol Neurosci N/A:N/A (2021). PubMed: 34125397
- Yarur HE et al. Functional Interplay of Type-2 Corticotrophin Releasing Factor and Dopamine Receptors in the Basolateral Amygdala-Medial Prefrontal Cortex Circuitry. Int J Neuropsychopharmacol 24:221-228 (2021). PubMed: 33125479
- Wysokinski A et al. Expression of Dopamine D1-4 and Serotonin 5-HT1A-3A Receptors in Blood Mononuclear Cells in Schizophrenia. Front Psychiatry 12:645081 (2021). PubMed: 33776821
- Cerantola S et al. Dopamine Transporter Genetic Reduction Induces Morpho-Functional Changes in the Enteric Nervous System. Biomedicines 9:N/A (2021). PubMed: 33923250
- Avchalumov Y et al. SCH23390 Reduces Methamphetamine Self-Administration and Prevents Methamphetamine-Induced Striatal LTD. Int J Mol Sci 21:N/A (2020). PubMed: 32899459