製品の概要

  • 製品名

    Anti-Dopamine D2 Receptor antibody
    Dopamine D2 Receptor 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Dopamine D2 Receptor
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab97357)

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 関連疾患

    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在

    Cell membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

画像

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Rat Substantia Nigra Lysate
    Lane 2 : Dopaminergic nucleus Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?
    Additional bands at: 52 kDa (possible glycosylated form)


    Exposure time: 4 minutes

プロトコール

参考文献

This product has been referenced in:

  • Lin CC  et al. Subchronic administration of aripiprazole improves fear extinction retrieval of Pavlovian conditioning paradigm in rats experiencing psychological trauma. Behav Brain Res 362:181-187 (2019). Read more (PubMed: 30610908) »
  • Beauvais G  et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). Read more (PubMed: 30366018) »
See all 7 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (AML3)
Permeabilization
Yes - 0.5% Triton
Gating Strategy
Live cells
Specification
AML3
Fixation
Formaldehyde

Abcam user community

Verified customer

投稿 Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y)
Permeabilization
Yes - TRITON X-100 (0'25%)
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 02 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

投稿 Feb 08 2012

Answer

Thank you for contacting us.  Unfortunately, I was unable to locate any amino acid sequences for Tilapia S100, S100 beta, or Dopamine D2 Receptor with which to compare the immunogen sequences for these antibodies.  For this reason, we have no information about whether or not these antibodies might react with Tilapia.  If the customer would still wish to test them, please let me know and I will be happy to set up testing discount codes. 

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