Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain slices (40um thick sections))
Specification
Brain slices (40um thick sections)
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Laboratories
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
I used paraformaldehyde (4%) fixed slices, embedded in agarose, cut on a vibrating microtome, applied the antibody in the presence of 0.3% Triton-X to ensure good permeabilisation of the membranes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 13 2006