Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

Submit a review Q&A (2)References (10)

Key features and details

  • Donkey Anti-Sheep IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot

製品の概要

  • 製品名

    Donkey Anti-Sheep IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details

  • 免疫原

    Full length native Sheep IgG (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    IHC image of Histone H3 staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab128012, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6900, 1/1000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

  • ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    ELISA results of purified ab6900 tested against purified Sheep IgG.

    Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (10)

ab6900 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6900 は 10 報の論文で使用されています。

  • Tomida S  et al. The SH3 domain in the fucosyltransferase FUT8 controls FUT8 activity and localization and is essential for core fucosylation. J Biol Chem 295:7992-8004 (2020). PubMed: 32350116
  • Seiron P  et al. Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced. J Pathol Clin Res 5:248-255 (2019). PubMed: 31493350
  • Svarcbahs R  et al. Removal of prolyl oligopeptidase reduces alpha-synuclein toxicity in cells and in vivo. Sci Rep 8:1552 (2018). PubMed: 29367610
  • Zhang L  et al. An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma. Front Immunol 9:511 (2018). PubMed: 29593741
  • Scott LL & Downing TG A Single Neonatal Exposure to BMAA in a Rat Model Produces Neuropathology Consistent with Neurodegenerative Diseases. Toxins (Basel) 10:N/A (2017). PubMed: 29286334
  • Svarcbahs R  et al. Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the a-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing a-Synuclein Oligomeric Species in Mouse Brain. J Neurosci 36:12485-12497 (2016). PubMed: 27927963
  • Durcan PJ  et al. Identification of novel Kirrel3 gene splice variants in adult human skeletal muscle. BMC Physiol 14:11 (2014). WB ; Human . PubMed: 25488023
  • Pelkonen A  et al. Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. J Neurochem 114:1168-76 (2010). PubMed: 20533994
  • Ziolkowska B  et al. alpha-Synuclein expression in the brain and blood during abstinence from chronic alcohol drinking in mice. Neuropharmacology 54:1239-46 (2008). WB . PubMed: 18456291
  • Norgett EE  et al. V1 and V0 domains of the human H+-ATPase are linked by an interaction between the G and a subunits. J Biol Chem 282:14421-7 (2007). PubMed: 17360703

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Question

Hi,
I recently purchased a Secondary Antibody to Sheep IgG - H&L (HRP)
(ab6900). It was delivered today but the solution looks cloudy and and
there are clearly visible particles floating in it. I have never bought
this particular product before, but I have never seen an antibody
solution like this before and am worried about the quality of the
antibody. I itend to use the antibody in a 3 day experiment and really
want to avoid wasting time and money on it. The batch number is
GR54072-1.
the order reference number is 1016507 and the purchase Order Number
462EWD. Would you mind having a look at this batch and sending a
replacement product, if possible?
Thanks,

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Answer

Thank you for contacting us.

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest pipetting up and down few times,vortexing the vial and using this antibody as normal. In our retesting experiment and from customer’s feedback we can say that ab is absolutely fine to use. So I would really encourage you to use the antibody first; If in case the antibody fails please do not hesitate to contact me for further assistance.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Kære Abcam,
We have today received the above order - 1 x 1 Donkey Polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)(Lot no. GR54072).
Unfortunately the antibody contained large precipitations which did not dissolve itself after mixing or after having been warmed-up to 37 degrees for 2-15 min. Please see the picture attached.
Please let us have a new antibody but please be sure that the new antibody is an antibody which is of good quality.
Do you want this one returned for some sort of examination ?

best regards







Med venlig hilsen/Best regards




________________________________

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Answer

Thank you for your inquiry.

It is normal to have brown precipitate form for HRP conjugates. The antibody that remains in solution is functional and the brown pellet should be discarded.

Irecommend to spin down the vial and try the antibody from the supernatantin your experiment. You might want to increase the amount you are using a little bit.

I would like to assureyou that if one of our products is not functioning as stated on the datasheet and was purchased with the last six month we will be happy to provide a free of charge replacement of credit note.

Please let me know if the antibody does not generate successful results after removing the precipitate.

Thank you for your cooperation.

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