Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)
Key features and details
- Donkey Anti-Sheep IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, IHC-P, ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Sheep IgG H&L (DyLight® 650)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Sheep -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
アプリケーション
適用あり: Flow Cyt, IHC-P, ICC/IFmore details -
標識
DyLight® 650. Ex: 654nm, Em: 673nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 650 replaces DyLight® 649. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96942の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/50 - 1/200.
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| IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
| ICC/IF |
1/50 - 1/500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/50 - 1/200. |
|
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
ICC/IF
1/50 - 1/500. |
画像
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)This image is courtesy of an Abreview submitted by Marcella Flores.
ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.
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Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96942 は 1 報の論文で使用されています。
- Esch MB et al. Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow. Lab Chip 15:2269-77 (2015). PubMed: 25857666