Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)

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Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)

    Key features and details

    • Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 488)
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 12nm Gold 6nm HRP HRP polymer Texas Red ® TRITC Unconjugated

    製品の概要

    • 製品名

      Donkey Anti-Sheep IgG H&L (DyLight® 488)
      IgG 二次抗体 製品一覧

    • 製品の詳細

      Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 488)

    • 由来種

      Donkey

    • ターゲット生物種

      Sheep

    • 特異性

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • アプリケーション

      適用あり: IHC-P, ICC/IF, Flow Cytmore details

    • 標識

      DyLight® 488. Ex: 493nm, Em: 518nm

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab96939の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    特記事項
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    画像

    • Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)
      Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab96939 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab96939 は 2 報の論文で使用されています。

    • Cen M  et al. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med 39:569-578 (2017). PubMed: 28204828
    • Kühn N  et al. Regulation of polar auxin transport in grapevine fruitlets (Vitis vinifera L.) and the proposed role of auxin homeostasis during fruit abscission. BMC Plant Biol 16:234 (2016). PubMed: 27793088

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    These are the antibodies that we have ordered in the recent past that don’t seem to work. My confocal data just shows a lot of background noise. Pl. help.

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    Answer

    According to my records, ab139923 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.



    Looking at our correspondence, it appears that we are awaiting protocol details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.



    If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.



    We wish you the best of luck with your research and look forward to a reply.

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    Question

    I wish to use this antibody for confocal microscopy. What is the recommended filter?

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    Answer

    We recommend using the filter used for FITC.

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    Question

    I have used this antibody to stain slides and get a signal, however it fades dramatically after just one pass through the confocal. Should this fade so quickly? Can I try another lot of antibody?

    Read More
    Answer

    Thank you for contacting Abcam regarding ab96939.


    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Hi,

    I have reviewed this profile. I might not clarify clearly what I need. I need to have the spectrum of the dye as you may know the excitation spectrum may cover a certain range not only at a certain point. I am doing multiple staining of different proteins so the overlap of either excitation or emission affect the result a lot. Can you please give me more details of your dye? (such as dye light). Thanks a lot!

    all the best

    Read More
    Answer

    Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

    I ma sorry there was a misunderstanding.

    We do have information on the spectra of DyLight® fluorochromes on our homepage:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12934

    The spectra can be reviewed on the bottom of the page. Unfortunately, we do not have a spectra viewer where multiple dyes can be combined and tested for overlapping. I am sorry if this causes any inconvenience.

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Maybe it is worth checking their website for a spectra viewer also.

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

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