Donkey Anti-Sheep IgG H&L (Biotin) (ab6899)

Submit a review Q&A (2)References (3)

Key features and details

  • Donkey Anti-Sheep IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot

製品の概要

  • 製品名

    Donkey Anti-Sheep IgG H&L (Biotin)
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details

  • 免疫原

    Full length native Sheep IgG (purified).

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • 特記事項（標識）

    Biotin/Protein Ratio: about 6 per IgG molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab6899 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6899 は 3 報の論文で使用されています。

  • Davis EA  et al. Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis. Exp Biol Med (Maywood) 242:1499-1507 (2017). PubMed: 28766984
  • Blackmore K  et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). PubMed: 28766983
  • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). PubMed: 25186745

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Can you please confirm the conjugation ratio of this antibody to biotin?

Read More
Answer

Merci pour votre appel et votre patience.

Je viens d'avoir la confirmation du laboratoire que effectivement le ratio de conjugaison de la biotine était de ˜6 moles de biotine par mole de IgG.

Je suis désolée pour cette erreur et je vous remercie d'avoir attiré notre attention à ce fait.

J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres questions.

Je vous souhaite un bon fin de semaine.

Read More

Question

any return information on this product for western or ihc? do you have the same product with fluorescent conjugates?

Read More
Answer

Thank you for your enquiry. We have yet to receive reviews from customers who have purchased and tested this antibody in WB or IHC. We also, don't have the same product with a fluorescent conjugate. To view a full list of our secondary antibodies, you can log onto our website and use the product browser. Please contact us again if you require further information to that provided on the datasheet.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com