Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (ab150180)
Key features and details
- Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
製品の概要
-
製品名
Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Sheep -
アプリケーション
適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
免疫原
The details of the immunogen for this antibody are not available.
-
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
-
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150180の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/200 - 1/1000.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
1/2000.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
1/200 - 1/1000. |
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
1/2000. |
画像
-
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (ab150180)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 2µg/ml) overnight at +4°C. Ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150180 Alexa Fluor® 594 donkey anti-sheep IgG (H+L) (orange) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (ab150180)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab150180 は 5 報の論文で使用されています。
- Dombrowski ME et al. Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress. Neurospine 17:69-76 (2020). PubMed: 32252156
- Larriva-Sahd J et al. On the existence of mechanoreceptors within the neurovascular unit of the mammalian brain. Brain Struct Funct 224:2247-2267 (2019). PubMed: 31190162
- Besse HC et al. Tumor Drug Distribution after Local Drug Delivery by Hyperthermia, In Vivo. Cancers (Basel) 11:N/A (2019). PubMed: 31600958
- Egunsola AT et al. Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal dysplasia. J Clin Invest 127:1475-1484 (2017). PubMed: 28263186
- Morimoto K et al. Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Endocrinology 157:1071-81 (2016). PubMed: 26789236