Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
Key features and details
- Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
製品の概要
-
製品名
Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WB, In-Cell ELISAmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster more details -
免疫原
Full length native protein (purified) corresponding to Rabbit IgG. Whole molecule.
-
標識
IRDye® 680RD
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Affinity chromatography purified using antigens coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab216779の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/25000.
|
|
| In-Cell ELISA |
1/200 - 1/800.
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/800. |
画像
-
Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)Lanes 1-3 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution (Anti-GAPDH antibody [6C5])
Lane 4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-?B p60 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
Lane 1 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216779)
Lanes 2-4 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab140751 overnight at 4°C. Antibody binding was detected using the Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
-
Spectrum - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab216779 は 1 報の論文で使用されています。
- Budden T et al. Ultraviolet light-induced collagen degradation inhibits melanoma invasion. Nat Commun 12:2742 (2021). PubMed: 33980846