Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

Submit a review Q&A (4)References (50)
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

製品の概要

  • 製品名

    Donkey Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000.
ELISA 1/160000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.

画像

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Canonical histones are retained in C. elegans sperm.

    A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
    For full details please see paper.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an anonymous Abreview.

    See Abreview

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an Abreview submitted by Min young Kim

    See Abreview

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    Lane M: 3 µl Molecular ladder.

    Lane 1: Rabbit IgG whole molecule.

    Lane 2: Rabbit IgG F(ab) fragment.

    Lane 3: Rabbit IgG F(c) fragment.

    Lane 4: Rabbit IgM whole molecule.

    Lane 5: Normal rabbit serum.

    All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.

    ab6802 used at 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (50)

ab6802 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6802 は 50 報の論文で使用されています。

  • Lei Z  et al. Dihydroartemisinin ameliorates dextran sulfate sodium induced inflammatory bowel diseases in mice. Bioorg Chem 100:103915 (2020). PubMed: 32450383
  • Lone AG & Bankhead T The Borrelia burgdorferi VlsE Lipoprotein Prevents Antibody Binding to an Arthritis-Related Surface Antigen. Cell Rep 30:3663-3670.e5 (2020). PubMed: 32187539
  • Jee JP  et al. Preparation and in vivo evaluation of a topical hydrogel system incorporating highly skin-permeable growth factors, quercetin, and oxygen carriers for enhanced diabetic wound-healing therapy. Int J Nanomedicine 14:5449-5475 (2019). PubMed: 31409998
  • Liu T  et al. Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa. Aging (Albany NY) 11:7880-7898 (2019). PubMed: 31548434
  • Xu S & Wu X miR-134 inhibits chondrogenic differentiation of bone marrow mesenchymal stem cells by targetting SMAD6. Biosci Rep 39:N/A (2019). PubMed: 30135141
View all Publications for this product

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

I recently bought this and found that the antibody solution is not clear, but is suspension. Is it normal and do I need to centrifuge it before use?

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Answer

It is normal that the solution is not completely clear. We recommend centrifuging the solution if it is still an unclear suspension after standing at room temperature (and gently mixed with some finger tapping).

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Question

What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

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Answer

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

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Answer

Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

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Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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