Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rabbit IgG, whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/3000.
|
|
| ELISA |
1/160000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/2000.
|
|
| IHC-P |
1/500 - 1/2000.
|
|
| IHC-Fr |
1/500 - 1/2000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/3000. |
|
ELISA
1/160000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/2000. |
|
IHC-P
1/500 - 1/2000. |
|
IHC-Fr
1/500 - 1/2000. |
画像
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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Canonical histones are retained in C. elegans sperm.
A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
For full details please see paper. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an anonymous Abreview. -
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an Abreview submitted by Min young Kim -
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) fragment.
Lane 3: Rabbit IgG F(c) fragment.
Lane 4: Rabbit IgM whole molecule.
Lane 5: Normal rabbit serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
ab6802 used at 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (76)
ab6802 は 76 報の論文で使用されています。
- Yang G et al. Hsa_circ_0008537 facilitates liver carcinogenesis by upregulating MCL1 and Snail1 expression via miR-153-3p. Oncol Rep 45:1072-1082 (2021). PubMed: 33469676
- Hao G et al. Copper-67 radioimmunotheranostics for simultaneous immunotherapy and immuno-SPECT. Sci Rep 11:3622 (2021). PubMed: 33574346
- Harris RA et al. Light chain myeloma and detection of free light chains in serum and urine of dogs and cats. J Vet Intern Med 35:1031-1040 (2021). PubMed: 33634914
- Jiang S et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
- Wang F et al. aB-Crystallin Alleviates Endotoxin-Induced Retinal Inflammation and Inhibits Microglial Activation and Autophagy. Front Immunol 12:641999 (2021). PubMed: 33777038