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Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

Submit a review Q&A (3)References (53)
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
  • ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB

製品の概要

  • 製品名

    Donkey Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Rabbit

  • 特異性

    Reacts with the heavy and light chains of the immunoglobulin molecule.

  • アプリケーション

    適用あり: WBmore details

  • 免疫原

    Synthetic peptide corresponding to IgG.

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項

    Part of the AbExcel range.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16284の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/20000. Optimized for ECL applications.
特記事項
WB
1/2000 - 1/20000. Optimized for ECL applications.

画像

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

    ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

    ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (53)

ab16284 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16284 は 53 報の論文で使用されています。

  • Castro-Gonzalez S  et al. HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner. Autophagy 17:553-577 (2021). PubMed: 32097085
  • Wang X  et al. Cryptotanshinone Ameliorates Doxorubicin-Induced Cardiotoxicity by Targeting Akt-GSK-3ß-mPTP Pathway In Vitro. Molecules 26:N/A (2021). PubMed: 33800264
  • Mazzola L  et al. Progressive Myoclonus Epilepsy Caused by a Homozygous Splicing Variant of SLC7A6OS. Ann Neurol 89:402-407 (2021). PubMed: 33085104
  • Li B  et al. miR-449a-5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33179102
  • Luo X  et al. Differential RNA packaging into small extracellular vesicles by neurons and astrocytes. Cell Commun Signal 19:75 (2021). PubMed: 34246289
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

WB with primary Ab to GAPDH (rabbit poly)
other secondary Abs work with this priamry
Ab: 1/10,000 - 1/2000 2h RT
block: 5% milk

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Answer

We have issued a free of charge replacement.

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Question

 I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards                                                    

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Answer

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

I purchased ab9071 and ab9072 as they are noted on the Abcam website to be useful as a matched pair for sELISA. However the anti-mouse HRP secondary is binding to the capture antibody as well as the mouse detection antibody, producing a very high signal even when no p24 is present.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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