Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7079)

Submit a review Q&A (2)References (2)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

製品の概要

  • 製品名

    Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • 追加情報
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab7079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7079 は 2 報の論文で使用されています。

    • Singh SJ  et al. Metabolic shift in density-dependent stem cell differentiation. Cell Commun Signal 15:44 (2017). PubMed: 29052507
    • Guo Z  et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Dear xxxxxx,

    Any chemical (for eg: glycerol) should be added to it when we aliquot it for freezing?

    Thank you for your prompt reply.

    Best regards,

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    Answer

    Thank you for your reply.

    Addition of glycerol to the antibody should not be required to maintain the activity of the antibody, however, if the customer wants to add glycerol please make them aware that glycerol can be contaminated with bacteria. If adding glycerol or any cryoprotectant, care should be taken to obtain a sterile preparation. Storing solutions containing glycerol at -80oC is not advised since this is below the freezing point of glycerol.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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    Question

    Dear Technical support,

    Can I get your advice on the storage method of this Donkey anti-Rabbit IgG H&L (FITC) secondary antibody" with cat number: ab7079?

    It is a FITC conjugated secondary antibody. The datasheet of the product recommend it to be store at -20°C or -80°C.

    Conjugated antibody stable to be store at -20C or -80C ? Freezing and thawing will not affect the conjugation?

    Thank you. We look forward to hearing from you soon.

    Best regards,

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    We would advise storing the Donkey anti-Rabbit IgG H&L (FITC) secondary antibody (ab7079) at +4°C short term (1-2 weeks). For longer term storage we would advise storing the antibody at -20°C or -80°C.We would advise avoid repeated freeze / thaw cycles as far as possible as this can affect the activity of antibodies. We would therefore recommend aliguoting the antibody before freezing.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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