Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
標識
DyLight® 488. Ex: 493nm, Em: 518nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96919の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/20000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/50 - 1/500.
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ICC/IF | (1) |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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WB
1/1000 - 1/20000. Predicted molecular weight: 36 kDa. |
IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
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rabbit anti-actin antibody at 1 µg/ml + HeLa whole cell lysate at 30 µg
Secondary
Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919) at 1/1000 dilution (Donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody)
Predicted band size: 36 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.
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Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)
E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.
This image is courtesy of an anonymous Abreview
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (15)
ab96919 は 15 報の論文で使用されています。
- Matthews AT et al. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int J Mol Sci 22:N/A (2021). PubMed: 34299212
- Li Q et al. M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis. Front Immunol 12:707784 (2021). PubMed: 34354711
- Ogino R et al. Adipose-Derived Stem Cells Promote Intussusceptive Lymphangiogenesis by Restricting Dermal Fibrosis in Irradiated Tissue of Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32485955
- Jara C et al. Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue. Diabetol Metab Syndr 12:66 (2020). PubMed: 32774470
- Si J et al. Antiepileptic effects of exogenous ß-hydroxybutyrate on kainic acid-induced epilepsy. Exp Ther Med 20:177 (2020). PubMed: 33101467