Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rabbit IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule. -
特記事項（標識）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab6801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/3000 - 1/15000. | |
| ELISA | 1/150000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/500 - 1/3000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
画像
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Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (10)
ab6801 は 10 報の論文で使用されています。
- Ding Q et al. miR-126 promotes the growth and proliferation of leukemia stem cells by targeting DNA methyltransferase 1. Int J Clin Exp Pathol 11:3454-3462 (2018). PubMed: 31949723
- Johnson MB et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . PubMed: 28634413
- Latorre R et al. Expression of the Bitter Taste Receptor, T2R38, in Enteroendocrine Cells of the Colonic Mucosa of Overweight/Obese vs. Lean Subjects. PLoS One 11:e0147468 (2016). PubMed: 26866366
- Ghorbani M et al. Apparent histological changes of adipocytes after treatment with CL 316,243, a ß-3-adrenergic receptor agonist. Drug Des Devel Ther 9:669-76 (2015). IHC-P . PubMed: 25709398
- Jung CS et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr . PubMed: 24663083
- Hoerder-Suabedissen A et al. Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia. Proc Natl Acad Sci U S A 110:3555-60 (2013). IHC-P . PubMed: 23401504
- Yin Y et al. Nuclear export factor 3 is involved in regulating the expression of TGF-ß3 in an mRNA export activity-independent manner in mouse Sertoli cells. Biochem J 452:67-78 (2013). PubMed: 23438076
- Hoerder-Suabedissen A & Molnár Z Molecular Diversity of Early-Born Subplate Neurons. Cereb Cortex : (2012). PubMed: 22628460
- Zhu M et al. Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage. Genome Biol 12:R77 (2011). IF . PubMed: 21846369
- Wei H et al. Bovine Herpesvirus Type 1 (BHV-1) U(L)49.5 Luminal Domain Residues 30 to 32 Are Critical for MHC-I Down-Regulation in Virus-Infected Cells. PLoS One 6:e25742 (2011). WB . PubMed: 22046246
