Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, ELISA, WB, IHC-P
製品の概要
-
製品名
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, ELISA, WB, IHC-Pmore details -
標識
Alkaline Phosphatase
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97061の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
|
| ELISA |
1/5000 - 1/50000.
|
|
| WB |
1/5000 - 1/50000.
|
|
| IHC-P |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
ICC
Use at an assay dependent dilution. |
|
ELISA
1/5000 - 1/50000. |
|
WB
1/5000 - 1/50000. |
|
IHC-P
1/100 - 1/1000. |
画像
-
Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
Lanes 3-4 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
Lanes 5-6 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab97061 は 6 報の論文で使用されています。
- Heidler TV et al. Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide. Commun Biol 4:106 (2021). PubMed: 33495563
- Jezek J et al. Synergistic repression of thyroid hyperplasia by cyclin C and Pten. J Cell Sci 132:N/A (2019). PubMed: 31331961
- Jezek J et al. Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment. EMBO Rep 20:e47425 (2019). PubMed: 31385392
- Molina-Aguilar C et al. Time-caloric restriction inhibits the neoplastic transformation of cirrhotic liver in rats treated with diethylnitrosamine. Carcinogenesis 38:847-858 (2017). PubMed: 28535183
- Kilinc S et al. Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage. Mol Cell Neurosci 74:58-70 (2016). PubMed: 26947098
- Mehrbod P et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . PubMed: 24788303
