Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750)
- Conjugation: Alexa Fluor® 750. Ex: 749nm, Em: 775nm
- Host species: Donkey
- Suitable for: WB
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WBmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 750. Ex: 749nm, Em: 775nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
特記事項
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750),ab175731 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 750), ab175731 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
関連製品
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Alternative Versions
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175651)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175780)
- Donkey Anti-Rabbit IgG H&L (ab182020)
- Donkey Anti-Rabbit IgG H&L (HRP) (ab205722)
- Donkey Anti-Rabbit IgG H&L (Biotin) (ab207999)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000.
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| 特記事項 |
|---|
|
WB
1/10000. |
画像
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Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175731 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
This data was developed using the unconjugated antibody (ab182020).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab175731 は 3 報の論文で使用されています。
- Benoit A et al. Flow cytometry for receptor analysis from ex-vivo brain tissue in adult rat. J Neurosci Methods 304:11-23 (2018). Flow Cyt . PubMed: 29660368
- Schwerdt HN et al. Cellular-scale probes enable stable chronic subsecond monitoring of dopamine neurochemicals in a rodent model. Commun Biol 1:144 (2018). PubMed: 30272020
- Zou Y et al. Notch 2 signaling contributes to cell growth, anti-apoptosis and metastasis in laryngeal squamous cell carcinoma. Mol Med Rep 14:3517-24 (2016). WB . PubMed: 27572051
