Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The section of formalin fixed human spinal cord was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins, then blocked in 5% BSA + 10% Horse serum. After incubation with primary antibody against IBA1, the section was incubated with Donkey anti-Rabbit Alexa fluor 594 from Abcam (1:400 in PBS) for 1 hr at room temperature.
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投稿 Jul 20 2017