Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed (ab7059)

Submit a review Q&A (2)References (3)

Key features and details

  • Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Donkey
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF

製品の概要

  • 製品名

    Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native mouse IgG (purified).

  • 標識

    Texas Red ®. Ex: 596nm, Em: 620nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000. PubMed: 18263878

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab7059 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7059 は 3 報の論文で使用されています。

  • Muller-Delp JM  et al. Effects of age and exercise training on coronary microvascular smooth muscle phenotype and function. J Appl Physiol (1985) 124:140-149 (2018). PubMed: 29025901
  • Planutis K  et al. A novel signaling pathway regulates colon cancer angiogenesis through Norrin. Sci Rep 4:5630 (2014). IHC-P ; Human . PubMed: 25005225
  • Hejna J  et al. Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway. J Biol Chem 283:9844-51 (2008). ICC/IF . PubMed: 18263878

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

human tissue
intestinal tumor as pos. crtl
no signal at all
4 um slides
AR: citrate buffer (heating plate)
block: 10% donkey serum 30min
ab16832: 5, 10 ug/ml
ab118519: 20 ug/ml

suggested: try other AR, use more Ab, try HRP-secondary + hematoxyline (to check if any signal can be obtained)

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Answer

Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8)
2)use more priamry antibody
3) try using an HRP-conjugated secondary antibody and hematoxyline (for nuclear staining)- to check if any signal can be obtained at all, with the experimentalsetup you have.

Right now it is difficult to determine where the problem might stem from - there could be multiple causes. Thus, please let me know what results you obtained after trying these tips and we can discuss this further.

I wish you good luck and look forward to hear back from you.

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Question

I have a mouse monoclonal which is IgG1kappa. I would like to make sure that the second antibody ab 7059 will recognise my monoclonal. could you please let me know?

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Answer

This antibody will detect IgG1 kappa.

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