Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (primary colon myofibroblasts)
Specification
primary colon myofibroblasts
Permeabilization
No
Fixative
Acetone
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA/PBS
Dilution
1/100
Secondary antibody
Name
Abcam antibody:Donkey anti-Mouse IgG H&L (DyLight 488) secondary antibody
Dilution
1/1000
Additional data
Additional Notes
Here costained with Vimentin (ab92547), Excellent staining!
Anti-alpha smooth muscle Actin- Green
Vimentin- Red
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Charles Pallangyo
Verified customer
投稿 Jul 03 2013