Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7062)

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Key features and details

  • Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Donkey
  • Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

製品の概要

  • 製品名

    Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native mouse IgG (purified).

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.

  • 特記事項（標識）

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • 追加情報
    Dot: Use at an assay dependant dilution.
    ELISA: 1/500 - 1/2000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab7062 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7062 は論文での使用が確認できていません。

    レビューと Q&A

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    Question

    Product code: 7062

    Inquiry: The customer wishes to use ab7062 as a secondary to ab24470. Do you predict some problem with it (since ab24470 is already conjugated to HRP)? Thanks in advance for your advise. Have a nice week.

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    Answer

    Thank you for your inquiry.
    There may be a problem in using the primary antibody ab24470 with a secondary antibody as it is already HRP conjugated itself. The steric hindrance created by this conjugate may hinder the efficient binding of the secondary antibody and may therefore lead to a reduced signal in the assay. As we have not tried this ourselves we cannot know exatly how badly the signal will be affected.
    I would recommend using an unconjugated antibody such as ab8315 or ab14729 as these will not lead to such problems.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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