Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150108)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Mouse -
アプリケーション
適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000.
ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control to complement this secondary antibody. |
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
1/2000. ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control to complement this secondary antibody. |
画像
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ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150108 Alexa Fluor® 594 donkey anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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ab16287 staining SSEA4 in rat stem cells derived from tendon by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with parafolmaldehyde, permeabilized with PBS containing 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hour at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG H&L (ab150108) (1/1000) was used as the secondary antibody.
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Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (112)
ab150108 は 112 報の論文で使用されています。
- Li Z et al. A novel peptide protects against diet-induced obesity by suppressing appetite and modulating the gut microbiota. Gut 72:686-698 (2023). PubMed: 35803703
- Zhu Z et al. The M1 muscarinic acetylcholine receptor regulates the surface expression of the AMPA receptor subunit GluA2 via PICK1. Psychopharmacology (Berl) 240:239-248 (2023). PubMed: 36564670
- Wu D et al. NRXN1 depletion in the medial prefrontal cortex induces anxiety-like behaviors and abnormal social phenotypes along with impaired neurite outgrowth in rat. J Neurodev Disord 15:6 (2023). PubMed: 36737720
- Yang H et al. Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery. JCI Insight 8:N/A (2023). PubMed: 37014712
- Selles MC et al. Oxytocin attenuates microglial activation and restores social and non-social memory in APP/PS1 Alzheimer model mice. iScience 26:106545 (2023). PubMed: 37128547