製品の概要

  • 製品名

    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種

    Donkey
  • ターゲット生物種

    Mouse
  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • アプリケーション

    適用あり: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details
  • 吸着処理血清


    Chicken, Cow, Goat, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab175700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.

画像

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175700 Alexa Fluor® 568 Donkey anti-Mouse IgG (H&L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

     

    Alexa Fluor® 568 is pseudocolored in yellow.

  • HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175700 Alexa Fluor® 568 Donkey anti-Mouse IgG (H&L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

     

    Alexa Fluor® 568 is pseudocolored in yellow.

  • Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.

参考文献

This product has been referenced in:

  • Ogata S  et al. Changes of Blood-Brain Barrier and Brain Parenchymal Protein Expression Levels of Mice under Different Insulin-Resistance Conditions Induced by High-Fat Diet. Pharm Res 36:141 (2019). Read more (PubMed: 31367840) »
  • Lautenschläger J  et al. C-terminal calcium binding of a-synuclein modulates synaptic vesicle interaction. Nat Commun 9:712 (2018). Read more (PubMed: 29459792) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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