Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)

Reviews (1)Q&A (4)References (6)
Dot Blot - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)
  • Flow Cytometry - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)

Key features and details

  • Donkey Anti-Goat IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

製品の概要

  • 製品名

    Donkey Anti-Goat IgG H&L (PE) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Goat

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details

  • 吸着処理血清


    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details

  • 免疫原

    Goat IgG whole molecule

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • 特記事項（標識）

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項

    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7004の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt (1)
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
特記事項
ICC/IF
1/100 - 1/250.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.

As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

画像

  • Dot Blot - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)
    Dot Blot - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)

    Dot Blot of ab7004.

    Antigen: Goat IgG.

    Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated.

    Secondary antibody: ab7004 at 1:1,000 for 60 min at RT.

    Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.

  • Flow Cytometry - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)
    Flow Cytometry - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)Verified Customer

    Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (6)

ab7004 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7004 は 6 報の論文で使用されています。

  • Arbore G  et al. Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism. Nat Commun 9:4186 (2018). PubMed: 30305631
  • Lan P  et al. TNF superfamily receptor OX40 triggers invariant NKT cell pyroptosis and liver injury. J Clin Invest 127:2222-2234 (2017). PubMed: 28436935
  • Mishra A  et al. Mechanism, Consequences, and Therapeutic Targeting of Abnormal IL15 Signaling in Cutaneous T-cell Lymphoma. Cancer Discov 6:986-1005 (2016). PubMed: 27422033
  • Rué L  et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J Clin Invest 126:4319-4330 (2016). PubMed: 27721240
  • Williams JW  et al. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun 4:2990 (2013). PubMed: 24356538
  • Miles A  et al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008). PubMed: 18308860

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Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.

Abcam user community

Verified customer

投稿 Sep 28 2015

Question

I need to know cross-reactivity for ab7004 (anti-goat IgG). Specialy-does the antibody react with swine (pig). Thanks for you time.

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Answer

Thank you for your enquiry. No reaction was observed with ab7004 against ant-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat serum proteins or other goat heavy or light chain proteins. Pig was not tested. If you have any further questions, please contact us again.

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Question

I read the datasheet and indeed I saw that the ab was tested for FACS. However I wondered whether it also was tested for INTRACELLULAR FACS. Because this procedure gives more problems with aspecific signals than normal FACS (extracellular) stainings.

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Answer

Thank you for your reply. Generally, if you intend to detect an intracellular protein via FACS analysis, you need to permeabilise the cells (i.e. using permeabilization buffer containing saponin) before the staining procedure. This secondary antibody should be suitable for recognizing both intracellular and extracellular epitopes. However, you must check the suitability of the primary antibody as well. Make sure that the primary and the secondary are compatible with each other and they both have been tested for FACS analysis.

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Question

PRE-SALES ENQUIRY Is the antibody also tested for intracellular staining (as sec ab) by means of flowcytometry? And do you have a protocol for this application?

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Answer

Thank you for your enquiry and your interest in our products. According to the datasheet, this secondary antibody (ab7004) has been tested for FACS/Flow cytometry. For details on general FACS analysis, please click on the “Protocols“ section on the right hand side panel of the Abcam homepage, you might find some useful information there.

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Question

What is the concentration of the Donkey antigoat IgG PE conjugated. I know you recomment 1:100- 1:200 dilution but i need to have the concentration. thanks

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Answer

We do not have a concentration for the antibody as such. However, the concentration of the label is 0.5 mg/ml, and we would estimate the antibody to be about 1 mg/ml.

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