Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

Submit a review Q&A (2)References (10)
Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

    Key features and details

    • Donkey Anti-Goat IgG H&L (HRP) preadsorbed
    • Conjugation: HRP
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

    製品の概要

    • 製品名

      Donkey Anti-Goat IgG H&L (HRP) preadsorbed
      IgG 二次抗体 製品一覧

    • 由来種

      Donkey

    • ターゲット生物種

      Goat

    • アプリケーション

      適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

    • 吸着処理血清


      Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

    • 免疫原

      Full length native Goat IgG (purified).

    • 標識

      HRP

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 6.50
      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 特記事項（標識）

      Horseradish Peroxidase (HRP)

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab7125の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/170000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ICC/IF
    1/500 - 1/2500.
    特記事項
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/170000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ICC/IF
    1/500 - 1/2500.

    画像

    • Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
      Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

      ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (10)

    ab7125 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7125 は 10 報の論文で使用されています。

    • Liu G  et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
    • Wu D  et al. Ameliorating effect of quercetin on epilepsy by inhibition of inflammation in glial cells. Exp Ther Med 20:854-859 (2020). PubMed: 32742328
    • Li L  et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
    • Nassar M  et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A 114:E337-E346 (2017). PubMed: 28049839
    • Rinaldi L  et al. Mitochondrial AKAP1 supports mTOR pathway and tumor growth. Cell Death Dis 8:e2842 (2017). IHC . PubMed: 28569781
    • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
    • Du Roure C  et al. Hematopoietic Overexpression of FOG1 Does Not Affect B-Cells but Reduces the Number of Circulating Eosinophils. PLoS One 9:e92836 (2014). WB . PubMed: 24747299
    • Mornet E  et al. Folate-equipped nanolipoplexes mediated efficient gene transfer into human epithelial cells. Int J Mol Sci 14:1477-501 (2013). PubMed: 23344053
    • Maekawa T  et al. Coiled-Coil Domain-Dependent Homodimerization of Intracellular Barley Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death. Cell Host Microbe 9:187-99 (2011). WB . PubMed: 21402358
    • Graham D  et al. Candidate genes that determine response to salt in the stroke-prone spontaneously hypertensive rat: congenic analysis. Hypertension 50:1134-41 (2007). WB . PubMed: 17938382

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    I want to inquire about the presence of kits of elisa IGG OF cat
    or conjugated of cat , and kits of IFAT IGG OF CAT or conjugated of cat
    You are kindly requested to respond as soon as possible
    I would also like to know the agent for the supply of this order in the Kingdom of Saudi Arabia
    thanks for time....

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    Answer

    Thank you for contacting us.



    Our distributor in Saudi-Arabia:

    Abdulla Fouad Holding Co.
    Abdulla Fouad Head Quarter Bldg.
    Dammam-Khobar Highway
    P.O. Box 257, Dammam
    31411
    Saudi Arabia
    Tel. +96626394455
    Fax. +96626396725
    http://www.abdulla-fouad.com



    We do not have kits for the detection of cat IgG, butI found anantibodywhich may interest you:

    https://www.abcam.com/Goat-Cat-IgG-Fc-ab112799.html (or use the following: https://www.abcam.com/Goat-Cat-IgG-Fc-ab112799.html).

    This antibody has been tested in ELISA and can be used with a secondary antibody like ab7125 Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed, to be used in an ELISA experiment.

    https://www.abcam.com/Donkey-Goat-IgG-HL-HRP-preadsorbed-ab7125.html (or use the following: https://www.abcam.com/Donkey-Goat-IgG-HL-HRP-preadsorbed-ab7125.html).

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    Question

    I have various normal human cell lines and tumor cell lines. My primary antibody is raised in goat. I am getting bands showing up in the negative control (secondary only). Looks like the secondary is binding non-specifically to the proteins in the lysates. I am using it at a 1/2000 dilution. I had the same problem with another secondary from another company, but this problem was cleared up by using one that had been pre-asorbed against human IgG. Has this secondary antibody been pre-adsorbed against human IgG, also?

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    Answer

    Ab7125 has only been pre-adsorbed to the following: Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. So, it most likely is binding non-specifically to proteins in your lysates. If you have any more questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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