Donkey Anti-Goat IgG H&L (HRP) (ab97110)
Key features and details
- Donkey Anti-Goat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: IHC-P, ELISA, WB, ICCmore details -
免疫原
Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituent: 0.05% CMIT/MIT based preservative -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項（標識）
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
関連製品
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Substrate reagent
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アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500.
|
|
| ELISA | (1) |
1/10000 - 1/100000. (Primary).
|
| WB | (3) |
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
|
| ICC |
1/200 - 1/500.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. |
|
ELISA
1/10000 - 1/100000. (Primary). |
|
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated. |
|
ICC
1/200 - 1/500. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/200 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab97110 は 39 報の論文で使用されています。
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- Clough B et al. C57BL/6 and 129 inbred mouse strains differ in Gbp2 and Gbp2b expression in response to inflammatory stimuli in vivo. Wellcome Open Res 4:124 (2019). PubMed: 31544161
- Touati SA et al. Cdc14 and PP2A Phosphatases Cooperate to Shape Phosphoproteome Dynamics during Mitotic Exit. Cell Rep 29:2105-2119.e4 (2019). PubMed: 31722221