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Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed (ab6951)

Submit a review Q&A (1)References (7)

Key features and details

  • Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed
  • Conjugation: Cy5.5 ®. Ex: 678nm, Em: 703nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated

製品の概要

  • 製品名

    Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Goat

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • 吸着処理血清


    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • 免疫原

    Full length native Goat IgG (purified).

  • 標識

    Cy5.5 ®. Ex: 678nm, Em: 703nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

  • 特記事項（標識）

    Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 4.1 moles Cy5.5 per mole of Donkey IgG

  • ポリ/モノ

    ポリクローナル

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6951の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (7)

ab6951 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6951 は 7 報の論文で使用されています。

  • Bauwens S  et al. The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin. Life (Basel) 11:N/A (2021). PubMed: 33804994
  • Hernandes MS  et al. Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain. J Neuroinflammation 15:45 (2018). PubMed: 29452577
  • Mendez-Bermudez A  et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
  • Wilhelm T  et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
  • Técher H  et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
  • Letessier A  et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320

レビューと Q&A

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Question

Any idea how long the Cy5.5 can be visualized after IHC staining procedure? Or does it just depend on length of time exposed to laser? What is best way to store sections stained with Cy5.5 conjugated secondary if I want to image in the future?

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Answer

Unfortunately we do not have data on the stability of C5.5 dyes on fixed stored IHC slides. This would depend on how they were imaged and the mounting medium that was used, as well as the exposure of the slides to light, however.
.

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